Analysis Note

Positive ControlRaji cells

Application

Flow Cytometry (1-10 µg/ml)r>Frozen Sections (see comments)r>Immunoblotting (see comments)r>Immunoprecipitation (see comments)r>Neutralization Studies (see comments)

General description

Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1) in Raji cells.

Protein A purified mouse monoclonal antibody. Recognizes D1 domain of the ~90 kDa CD54 protein (ICAM-1).

Anti-CD54, mouse monoclonal, clone 15.2, recognizes D1 domain of the ~90 kDa CD54 (ICAM-1) in Raji cells. It is validated for FC, WB, IP, IHC on frozen sections, and neutralization studies.

Immunogen

Human

human monocytes

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

FACS was performed on 5x10⁵ cultured Raji human tumor cells using 1 µg/ml Anti-CD54 and FITC-conjugated goat anti-mouse IgG. This antibody will inhibit CD54 binding to LFA-1. This antibody has also been reported to work for frozen sections, immunoblotting, and immunoprecipitation. Variables associated with assay conditions will dictate the proper working dilution.

Reilly, P.L., et al. 1995. J. Immunol. 155, 529.Berendt, A.R., et al. 1992. Cell68, 71.Dransfield, I., et al. 1992. J. Cell Biol.116, 1527.

Packaging

100 µg in Plastic ampoule

Physical form

In 150 mM NaCl, 100 mM KCl, 50 mM sodium phosphate, pH 7.5.

Reconstitution

Following initial use, aliquot and freeze (-20°C) for long-term storage. Avoid freeze/thaw cycles.

Warning

Toxicity: Standard Handling (A)